Journal: PLoS ONE

Article Title: A Rapid Subtractive Immunization Method to Prepare Discriminatory Monoclonal Antibodies for Food E. coli O157:H7 Contamination

doi: 10.1371/journal.pone.0031352

Figure Lengend Snippet: Bacterial strains used in the study.

Article Snippet: The following strains were tested including Salmonella sp. (CMCC 50325), Citrobacter freundii (IHEM 1.5001), Staphylococcus aureus (CMCC 29213), Shigella (CMCC 51081,51066), Non-O157 E. coli (CMCC 44216, 44505), E. coli O157:H19 (CMCC 44752), E. coli O157:H7 (ATCC 43895).

Techniques:

Journal: PLoS ONE

Article Title: A Rapid Subtractive Immunization Method to Prepare Discriminatory Monoclonal Antibodies for Food E. coli O157:H7 Contamination

doi: 10.1371/journal.pone.0031352

Figure Lengend Snippet: A, sera from Cy-treated mice (experimental group) and no Cy-treated mice (control group) were diluted and tested for antibody reactivity to E. coli O157:H19 in whole cell ELISA (OD 450 nm); B, sera were diluted and tested for reactivity to E. coli O157:H7 and E. coli O157:H19 (▪: experimental group; •: control group).

Article Snippet: The following strains were tested including Salmonella sp. (CMCC 50325), Citrobacter freundii (IHEM 1.5001), Staphylococcus aureus (CMCC 29213), Shigella (CMCC 51081,51066), Non-O157 E. coli (CMCC 44216, 44505), E. coli O157:H19 (CMCC 44752), E. coli O157:H7 (ATCC 43895).

Techniques: Control, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: A Rapid Subtractive Immunization Method to Prepare Discriminatory Monoclonal Antibodies for Food E. coli O157:H7 Contamination

doi: 10.1371/journal.pone.0031352

Figure Lengend Snippet: Summary of the characteristic of some mAbs reacting with Escherichia coli O157:H7 and others.

Article Snippet: The following strains were tested including Salmonella sp. (CMCC 50325), Citrobacter freundii (IHEM 1.5001), Staphylococcus aureus (CMCC 29213), Shigella (CMCC 51081,51066), Non-O157 E. coli (CMCC 44216, 44505), E. coli O157:H19 (CMCC 44752), E. coli O157:H7 (ATCC 43895).

Techniques:

Journal: PLoS ONE

Article Title: A Rapid Subtractive Immunization Method to Prepare Discriminatory Monoclonal Antibodies for Food E. coli O157:H7 Contamination

doi: 10.1371/journal.pone.0031352

Figure Lengend Snippet: The characteristics of mAbs against E. coli O157:H7.

Article Snippet: The following strains were tested including Salmonella sp. (CMCC 50325), Citrobacter freundii (IHEM 1.5001), Staphylococcus aureus (CMCC 29213), Shigella (CMCC 51081,51066), Non-O157 E. coli (CMCC 44216, 44505), E. coli O157:H19 (CMCC 44752), E. coli O157:H7 (ATCC 43895).

Techniques:

Journal: PLoS ONE

Article Title: A Rapid Subtractive Immunization Method to Prepare Discriminatory Monoclonal Antibodies for Food E. coli O157:H7 Contamination

doi: 10.1371/journal.pone.0031352

Figure Lengend Snippet: E. coli O157:H7 cell lysates were subjected to SDS–PAGE, transferred to nitrocellulose filter, and probed with 1C6 (lane 1), 1D8, (lane 2), 4A7 (lane 3), 5A2 (lane 4), 5D8 (lane 5). The sizes of molecular weight standards are shown at the right (lane 6).

Article Snippet: The following strains were tested including Salmonella sp. (CMCC 50325), Citrobacter freundii (IHEM 1.5001), Staphylococcus aureus (CMCC 29213), Shigella (CMCC 51081,51066), Non-O157 E. coli (CMCC 44216, 44505), E. coli O157:H19 (CMCC 44752), E. coli O157:H7 (ATCC 43895).

Techniques: SDS Page, Molecular Weight

Journal: PLoS ONE

Article Title: A Rapid Subtractive Immunization Method to Prepare Discriminatory Monoclonal Antibodies for Food E. coli O157:H7 Contamination

doi: 10.1371/journal.pone.0031352

Figure Lengend Snippet: Immune-colloidal gold probes coated with mAb 5A2 were applied to special single out E. coli O157:H7 contaminated minced beef from others which included food samples contaminated by ATCC 43895 (A1,B1,C1,D1), IHEM 1.3001 (A2), IHEM 1.3002 (A3), IHEM 1.3003 (A4), CMCC 50303 (B2), CMCC 50115 (B3), CMCC 50309 (B4), CMCC 51135 (C2), CMCC 51081 (C3), CMCC 51066 (C4), CMCC 44102 (D2), CMCC 44109 (D3), CMCC 44156 (D4). Negative control A5, B5, C5, D5) refers to food samples which have no bacterial contamination.

Article Snippet: The following strains were tested including Salmonella sp. (CMCC 50325), Citrobacter freundii (IHEM 1.5001), Staphylococcus aureus (CMCC 29213), Shigella (CMCC 51081,51066), Non-O157 E. coli (CMCC 44216, 44505), E. coli O157:H19 (CMCC 44752), E. coli O157:H7 (ATCC 43895).

Techniques: Negative Control

Journal: PLoS ONE

Article Title: Shiga Toxin-Mediated Hemolytic Uremic Syndrome: Time to Change the Diagnostic Paradigm?

doi: 10.1371/journal.pone.0001024

Figure Lengend Snippet: Comparison of putative virulence and fitness genes of stx -negative and stx -positive E. coli strains of serotypes O26:H11/NM, O103:H2/NM, O121:H19, O145:H28/NM, and O157:H7/NM.

Article Snippet: To identify non-O157 E. coli strains, non-separated broth cultures were inoculated onto SMAC and enterohemolysin agar (Sifin, http://www.sifin.de ).

Techniques: Comparison

Journal: PLoS ONE

Article Title: Shiga Toxin-Mediated Hemolytic Uremic Syndrome: Time to Change the Diagnostic Paradigm?

doi: 10.1371/journal.pone.0001024

Figure Lengend Snippet: Comparison of phenotypes of stx -negative and stx -positive E. coli strains of serotypes O26:H11/NM, O103:H2/NM, O121:H19, O145:H28/NM, and O157:H7/NM.

Article Snippet: To identify non-O157 E. coli strains, non-separated broth cultures were inoculated onto SMAC and enterohemolysin agar (Sifin, http://www.sifin.de ).

Techniques: Comparison

Journal: PLoS ONE

Article Title: Crystal Structure of ORF210 from E. coli O157:H1 Phage CBA120 (TSP1), a Putative Tailspike Protein

doi: 10.1371/journal.pone.0093156

Figure Lengend Snippet: (A) Analytical gel filtration of purified TSP1 (red). Predicted monomer is 82 kDa and a trimer is 246 kDa. Gel filtration standards (blue) are as follows: (1) Thyroglobulin, 670 kDa (void volume); (2) Gamma globulin, 158 kDa; (3) Ovalbumin, 44 kDa; (4) Myoglobin, 17 kDa; (5) Vitamin B 12 , 1.35 kDa. (B) Thermal unfolding monitored by CD spectroscopy. The experimental values are indicated by+symbols and the continuous line corresponds to the theoretical curve. (C) TSP1 SDS and protease stability. Lanes: (1) BSA; (2) BSA+trypsin; (2) BSA+chymotrypsin; (4) TSP1 boiled; (5) TSP1 non-boiled; (6) TSP1+trypsin, boiled; (7) TSP1+trypsin, non-boiled; (8–9) As with 6–7 but with chymotrypsin. (D) Immuno-dot blot. (1) E. coli ATCC 35218 (Non-O157:H7); (2) E. coli DH5α (Non-O157:H7); (3) E. coli ATCC 700728 (O157:H7); (4) E. coli ATCC 43894 (O157:H7); (5) O157 LPS from ATCC 43894; (6) O157 LPS from List Biologicals; (7) K. pneumoniae ATCC 700603; (8) His-tagged TSP1 (positive control); (9) Buffer (negative control); (10) empty; (11) PlyCB (negative control protein); (12) His-tagged PlyCB (positive control protein).

Article Snippet: E. coli O157:H7 strains (ATCC 43894 and 700728), non-O157 E. coli strains (ATCC 35218, DH5α), and Klebsiella pneumonia (ATCC 700603) were grown overnight and 4 μL of each were spotted on a nitrocellulose membrane (Ambion).

Techniques: Filtration, Purification, Circular Dichroism, Dot Blot, Positive Control, Negative Control

Journal: Microorganisms

Article Title: Characterization of Non-O157 Escherichia coli from Cattle Faecal Samples in the North-West Province of South Africa

doi: 10.3390/microorganisms7080272

Figure Lengend Snippet: Phenotypic resistance and antimicrobial resistance genes associated with non-O157 E. coli isolates.

Article Snippet: Briefly, agarose-embedded DNA of non-O157 E. coli was digested for 2 h with 20 units of XbaI restriction enzyme (New England Biolabs, Pickering, ON, Canada).

Techniques:

Journal: Microorganisms

Article Title: Characterization of Non-O157 Escherichia coli from Cattle Faecal Samples in the North-West Province of South Africa

doi: 10.3390/microorganisms7080272

Figure Lengend Snippet: Biofilm formation by non-O157 STEC on a polystyrene surface at 22 °C ( A ) and 37 °C ( B ) using M9 medium. The vertical axis represents the median OD 590 nm of eight replicates of each strain. Horizontal lines represent the cut-off values between weak, intermediate and strong biofilm producers. The OD is defined as three standard deviations above the mean OD of the negative control. Strains were classified as follows: OD ≤ ODc (0.082), non-adherent; ODc < OD ≤ 2 X ODc, weakly adherent; 2 X ODc < OD ≤ 4 X ODc, moderately adherent; 4 X ODc < OD strongly adherent. OD, optical density; ODc, cut-off OD value.

Article Snippet: Briefly, agarose-embedded DNA of non-O157 E. coli was digested for 2 h with 20 units of XbaI restriction enzyme (New England Biolabs, Pickering, ON, Canada).

Techniques: Negative Control

Journal: Microorganisms

Article Title: Characterization of Non-O157 Escherichia coli from Cattle Faecal Samples in the North-West Province of South Africa

doi: 10.3390/microorganisms7080272

Figure Lengend Snippet: Biofilm-forming ability of non-O157 E. coli isolates on polystyrene and number of isolates positive for biofilm forming genes in isolates that were positive for Shiga toxin genes and multi-drug resistant non-STEC.

Article Snippet: Briefly, agarose-embedded DNA of non-O157 E. coli was digested for 2 h with 20 units of XbaI restriction enzyme (New England Biolabs, Pickering, ON, Canada).

Techniques:

Journal: Microorganisms

Article Title: Characterization of Non-O157 Escherichia coli from Cattle Faecal Samples in the North-West Province of South Africa

doi: 10.3390/microorganisms7080272

Figure Lengend Snippet: Pulsed-field gel electrophoresis dendrogram of E. coli non-O157, strains isolated from cattle (beef and dairy) faecal samples from different locations in the North-West Province of South Africa. E. coli genomic DNA was digested with XbaI and the dendrogram was constructed using an unweighted pair-group method.

Article Snippet: Briefly, agarose-embedded DNA of non-O157 E. coli was digested for 2 h with 20 units of XbaI restriction enzyme (New England Biolabs, Pickering, ON, Canada).

Techniques: Pulsed-Field Gel, Electrophoresis, Isolation, Construct

Journal: Microorganisms

Article Title: Characterization of Non-O157 Escherichia coli from Cattle Faecal Samples in the North-West Province of South Africa

doi: 10.3390/microorganisms7080272

Figure Lengend Snippet: Sequence annotation results for non-O157 E. coli serotype determinants (O- and H-antigen sequences) and stx genes.

Article Snippet: Briefly, agarose-embedded DNA of non-O157 E. coli was digested for 2 h with 20 units of XbaI restriction enzyme (New England Biolabs, Pickering, ON, Canada).

Techniques: Sequencing